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how to get the C-slow signaling?
Amplifier: HEKA EPC9
Software: Patchmaster 2.05
Question:
when the cell membrane been ruptured, there would be a C-slow signal, how can I get this signal and send it to a single chip or another computer?
Answer:
| Bernd Letz (support@heka.com) |
| If you are using an EPC 9 or EPC 10 amplifier, then you can output the capacitance and G-series values as voltages on the DA channels 0 and 1 of the amplifier. This can be done by selecting ´Output Cap. Track´ from the EPC 9 menu or EPC 10 menu. If selected, the C-slow value is available at DAC 0, and the G-series value (1/R-series) is available at DAC 1. The scaling for G-series is 100 nS/V; the scaling of C-slow is 0.5, 5 or 50 pF/V for the 30, 100 and 1000 pF range, respectively. The DAC values will be reset to zero when activating Reset. Therefore, make sure that the trigger DAC (to be set in the Configuration window) is neither DAC 0 nor DAC 1 when you want to activate Output Cap. Track. Don´t forget to turn of the ´Cap Track´ in the amplifier dialog. Otherwise the capacitance and G-series values are not updated. |
不过我软件上显示的Output Cap. Track是灰色,这个问题还没有解决.实时看Rs和C-SLOW还是可以的.
Note:
The procedure for estimating the CSlow and Rs value is described in detail in the paper ´Design of the EPC-9, a computer-controlled patch clamp amplifier. 2. Software´ written by Sigworth, Affolter and Neher (J. of Neurosc. Methods 65, 1995, 203-215).
